. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5 microplate reader. For highly selective detection of dsDNA over RNA, even in the presence of contaminants. 2 to 1000 ng) Assay Kit and Qubit dsDNA HS (high sensitivity, 0. The kits provide concentrated assay reagent, dilution buffer, and prediluted DNA standards. 2000 ng/mL of lamda dsDNA was serially diluted in 1XTE before the addition of the PicoGreen working solution. 50 pg to 2 µg. 1 Active esters and kits for labeling proteins and. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. SYPRO and Coomassie Fluor luminescent and fluorescent protein gel stains—Table 9. ZERO BIAS - scores, article reviews, protocol conditions and morePicogreen assay with Qubit platform is considered to be the method of choice for DNA quantification. the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Unit Size. 2mL 1 x 96 wellsAssay Plate with Plate Sealing Foil, D002 *Component can be purchased separately. • Specific for dsDNA in the presence of RNA. Using this data as a starting point, the optimal conditions (e. No. Picogreen assays are compatible with most microplate readers and fluorometers. Unit Size. Catalog Number Specifications Unit Size Product Type Quantitation Range Packaging Type Price (EUR) Availability Quantity;DNA Quantification Using Quant-iT™ PicoGreen® The Quant-iT™ PicoGreen® assay was tested to overcome limitations of the UV absorbance assay at very low DNA concentrations. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay for nucleic acid quantification. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 反应次数. High tolerance for contaminants. Contents and Storage Information. Cloning, expression and proteins purification. 50 pg to 2 µg. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). Product Type. Free dye. • Quant-iT dsDNA Assay Kit, high sensitivity (Cat. Picogreen assays are compatible with most microplate readers and fluorometers. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. ZERO BIAS - scores, article reviews, protocol conditions and moreSelectively detect dsDNA from a wide variety of sources, and even in presence of ssDNA, RNA, and free nucleotides, with the Quant-iT PicoGreen dsDNA Assay Kit and PicoGreen dsDNA Reagent. Quant-iT PicoGreen dsDNA Assay Kits and Reagents are designed to selectively detect dsDNA from a wide variety of sources, even in presence of ssDNA, RNA, and free. Quant-iT™ PicoGreen® dsDNA Assay Kit *2,000 assays* (includes PicoGreen® Reagent, 10X buffer, and standard λ DNA) Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 10 ng/µl to 100 ng/µl RNA (>500 bp; not specific for RNA over DNA) RNase protection assays Microarray samplesIn this research six cyanine fluorophores for the quantification of dsDNA in the pg-ng range, without amplification, are compared under exactly identical conditions: EvaGreen, SYBR Green, PicoGreen, AccuClear, AccuBlue NextGen and YOYO-1. Microplate reader setup for a Quant-iT 1X dsDNA HS Assay. . Pico Green dsDNA Assay 2 03/04/2019The assay was performed according to the initial MPO-DNA complex ELISA protocol, but blocking buffer, sample diluent and detection antibody diluent were replaced by an in-house blocking buffer. Unlike many other DNA quantitation methods, PicoGreen is minimally affected by the presence of lipid, protein, carbohydrates and other cellular. Invitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kits and dsDNA Reagents. P7589) was used to quantify DNA by fluorescence. The superior sensitivity and performance of the Modulus™ Microplate Fluorometer allows for dsDNA detection as low as 250ng/mL. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267dsDNA in the presence of equimolar concentrations of ssDNA and RNA with minimal effect on the quantitative results. Calibrator MPO. Figure 2. BR reagent (Component A) 1. Common contaminants such as salts, free nucleotides, solvents,Product Description. Rapidly detect as little as 0. QuantiFluor® ONE dye sensitivity range is 0. quant it picogreen dsdna quantitation assay kit - by Bioz Stars , 2023-09. 适用于（设备）. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. Description. Q33120). 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Breadcrumb. The kit contains: • PicoGreen dsDNA quantitation reagent, 1 ml, stock solution in DMSO • 20 x TE buffer, 25 ml, 200 mM 製品番号（カタログ番号）： P11495. 19. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. In the Quant-iT/PicoGreen assay, DNA and working solution were mixed 1:1. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Application observe demonstrating so with Molecular Devices SpectraMax® microplate book and the Quant-iT PicoGreen assay, users can reliably size concentrations as low as 50 pg/mL of double-stranded DNA. The use of a serial dilution of dsDNA standards whileThe quantity of ChIP DNA should first be determined with the Quant-iT™ PicoGreen dsDNA Reagent Kit (Invitrogen). • Suitable for use in 96- and 384-well plate formats. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. 1 Warm Quant-iT PicoGreen reagent to room temp in the dark. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. Compared with the aforementioned methods, the PicoGreen dye binding assay for dsDNA has greater sensitivity, specificity, and linear range. Quant-iT ™ PicoGreen ™ dsDNA Assay Kit 1 mL assay kitlimit consume only 2 picograms of dsDNA per measure-ment. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. AccuBlue® RNA Quantitation Kit Details. (dsDNA) High Sensitivity (HS) assay, Quant-iT PicoGreen Assay, Bioanalyzer HS DNA assay, and NanoDrop Onec. assay or add 5 µL Quant-iT ™ RiboGreen ™ RNA Reagent to 9. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 产品概述. 2. Mit den Quant-iT Picogreen dsDNA-Assay-Kits und dsDNA-Reagenzien erzielen Sie präzise und präzise dsDNA-Konzentrationsmessungen über einen breiten dynamischen Bereich. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 5 mm in the Eppendorf BioSpectrometer fluorescence, PicoGreen reagent is diluted in dimethylsulfoxide (DMSO) which solidifies at refrigerator temperatures. Unit Size. Product Type. Quantitation Range. samples as well as standard in the assay itself. Quantifications using the UVette require half the amount ofCell viability was measured using a WST-8 assay (Cell Counting Kit-8, Dojindo). The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. Catalog Number. Catalog Number. Mix by gently vortexing for 5 seconds. 5) prepared from 20x. Reagents and materials PicoGreen dsDNA Quantitation Kit, Invitrogen Molecular Probes, #P-7589 or equivalent. Picogreen assays are compatible with most microplate readers and fluorometers. Libraries were sequenced on the Illumina NovaSeq. 2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. Picogreen assays are compatible with most microplate readers and fluorometers. 双链DNA（dsDNA）定量检测试剂盒 【产品升级声明】： 我司（懋康生物）从22. HS Assay Kit or Quant-iT™ PicoGreen® dsDNA Assay Kit. 22. In addition to the Quant-iT™ dsDNA High-Sensitivity Assay Kit described here, we also offer the Quant-iT ™ dsDNA Broad-Range Assay Kit (Cat. (A) The overall set up of the assay includes manual preparation of the reagents, automated preparation of the assay plate and fluorometric quantification using a plate reader. (Left) The Quant-iT dsDNA Assay Kit, High Sensitivity has a linear detection range of 0. PicoGreen reagent provides rapid and easy determination of dsDNA, offering a wide dynamic range. Rapidly detect as little as 0. 9 Q33230 9 500 28. The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. Mix PicoGreen and DNA by pippetting up and down 5-10 times. Quant-iT PicoGreen dsDNA Assay Kit and Reagent (Ex/Em = 502/523 nm) dsDNA: 0. 1X dsDNA assay (%) 100 17. The sample must yield a minimum of 55 ng of genomic DNA to ensure sufficient DNA for quality control (QC) and. The PicoGreen dsDNA quantitation reagent and kits are ideal for PCR-based assays, microarray samples, DNA damage assays, enzyme activity assays, genomic DNA. P7589. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Table 2 summarizes results of the Quant-iT Prepare the first standard Combine 20uL of 100ug/mL lambda DNA from the Quant-IT PicoGreen dsDNA assay kit (Fisher P7589) with 980uL 1X TE to create a 2ug/mL standard. For example, 3 µL of the DNA standard mixed with 147 µL of TE is sufficient for the standard curve described in step 2. PicoGreen®試薬アッセイは、蛍光を基にした迅速かつ簡単な手法により、dsDNAを正確に定量することができます。. Thermo Fisher quant it picogreen dsdna assay kit. Nucleic acid quantitation using Quant-iT Reagents. Using the Quant-iTTM PicoGreen® dsDNA reagent and the recommended assay protocol, researchers can quantitate dsDNA in the presence of equi molar concentrations of ssDNA and RNA with minimal effect on the quantitation results. Prepare a 1X TE working solution by diluting the concentrated buffer 20-fold with sterile, distilled, DNase-free water. PicoGreen dsDNA quantitation reagents are. The assay was prepared for 184 aliquots of DNA sample and five DNA standards, including one blank. This protocol is a simplified and condensed. To perform fluorometric quantification of cfDNA quantity Quant-iT PicoGreen dsDNA Assay Kit (Thermofisher), was used, following the manufacturer’s instructions. Helixyte™ Green dsDNA Quantitation Assay Kit is a few magnitudes more sensitive than UV absorbance readings. quant it picogreen dsdna assay kit , 2023-10. DNA standards ranging from 1000 ng/mL to 50 pg/mL were assayed using the Quant-iT PicoGreen dsDNA Assay and SpectraMax M5. Description. P11495. (2012). Significantly increased sensitivity over absorbance at 260nm (NanoDrop® spectrophotometer) for those samples that are low in concentration. For Research Use Only. 2–100 ng: 2000: O11492 O7582: Quant-iT OliGreen ssDNA Assay Kit and Reagent (Ex/Em = 498/518 nm) ssDNA: 1–200 ng: 2000: R11490 R11491: Quant-iT RiboGreen RNA Assay Kit and Reagent (Ex/Em = 500/525 nm) RNA: 20–1000 ng: 2000: Q32883:DNA concentration was quantified with Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) on a Tecan plate reader. Picogreen assays are compatible with most microplate readers and fluorometers. 0 fluorometer and Quant-iT PicoGreen dsDNA. For example, if the assay requires 50 wells, add 600 L of PicoGreen to 14. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was added to 199 μl of a Qubit working solution. The QuantiFluor® dsDNA dye offers greater sensitivity over absorbance at 260nm (NanoDrop) low-concentration samples, and better or equal sensitivity performance when compared to Quant-IT™ dsDNA BR. 99 to $1999. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267DNA concentration was measured using a spectrometer with the Quant-iT PicoGreen dsDNA Assay Kit (Thermo Fisher Scientific). The accuracy index is defined in Table 2. The Quant-iT dsDNA High-Sensitivity Assay Kit and the Quant-iT dsDNA Broad-Range Assay Kit make DNA quantification easy and accurate. 9. The Quant-iT PicoGreen dsDNA Assay Kit from Thermo Fisher Scientific is more specific for DNA and is about 1000 times more sensitive than traditional absorbance methods. 95) in the range of 10 pg till 100 ng for the salmon dsDNA as well as for the control dsDNA from the kit (λ dsDNA standard) (Fig. PicoGreen dsDNA quantitation reagents are. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. 2. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. 2. Technical note | Quant-iT and Qubit 1X dsDNA assays the Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. 5), 200X PicoGreen. Control samples were not lysed and consisted of free pDNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. 2. Both the Quant-iT High-Sensitivity dsDNA Assay Kit and the Quant-iT Broad-Range dsDNA Assay Kit provide concentrated assay reagent, dilution buffer, and pre-diluted DNA standards. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). The lambda DNA standard, provided at 100 µg/mL in the Quant-iT ™ PicoGreen dsDNA Assay Kit, is diluted 50‑fold in TE to make the 2 µg/mL working solution. 0 ml) of the aqueous working solution of Quant-iT™ PicoGreen dsDNA Reagent was added to each cell sample, which was incubated for 10 min at 37 °C in the dark. Quant-iT PicoGreen dsDNA assay The Quant-iT PicoGreen dsDNA assay was performed according to the kit instructions. quant it picogreen dsdna assay kit reagents ( Thermo Fisher ) 86. This protocol is a simplified and condensed version of. Picogreen Reagent b. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 5). The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0. PicoGreen定 量检测方法简单、方便，被多家生物制品厂所选择，成为生物制品残留DNA检测的标准。 PicoGreen与DNA双链结合后才发出的荧光，无DNA不发荧光；所发荧光与DNA浓度成 正比。在2010年《中国药典》中提出，PicoGreen定量DNA的方法检出限约0. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). The Qubit dsDNA BR (Broad-Range) Assay Kit, when used with the Qubit Fluorometer, provides an accurate and selective method for the quantitation of DNA samples. Quant-iT™ PicoGreen dsDNA Assay Kit (Life Technologies; Cat. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Buy from Supplier. ただ、この製品のマニュアルには蛍光分光光度計での測定方法しか. Analysis of cfDNA and H3Cit was performed using Quant-iT™ PicoGreen ® dsDNA Assay Kit or an ELISA, respectively. If you would like to use this kit with the Qubit® fluorometer, we have includedconjunction with Molecular Probes' PicoGreen dsDNA Quantitation Reagent enables researchers to quantitate as little as 1 ng/mL of dsDNA. Quant-iT PicoGreen dsDNA kit by Thermo Scientific Catalog #: P7589 2Prepare 1XTE buffer from 20X stock solution using nuclease-free water: will need 200 μl/well (for. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. No. DNA standards (Component C) set of 8, 500 µl each (0, 5, 10, 20, 40, 60, 80, 100 ng/µl) 2. 2. 2–400ng/µl (1µl sample in 200µl assay). Protocols All Picogreen reactions and measurements werethe Quant-iT™ PicoGreen® dsDNA Assay Kits is certified to be nucleic acid–free and DNase-free. Selectively detect dsDNA from a wide variety of sources, and even in presence of. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. This protocol is a simplified and condensed version of. 0). DNA samples with a concentration determined by 260 nm absorbance higher than 100 ng/mL were diluted. Material Amount Concentration Storage Stability Quant-iT™ PicoGreen® dsDNA reagent (Component A) 1 mL in 1 vial (P7589) or in10 vials of 100 μL each (P11496) Solution in DMSO 2–6ºC Desiccate Protect from light • • • When stored as directed, product stable. Related applications: Nucleic Acid Quantitation. Quant-iT™ PicoGreen® dsDNA Assay Kit *10 x 100 µl* 1 kit P11496 Quant-iT™ PicoGreen® dsDNA Reagent 1 kit P7581 Quant-iT™ PicoGreen® dsDNA Reagent *10 x 100 µl* 1 kit P11495 Quant-iT™ RiboGreen® RNA Reagent *2,000 assays* 1 kit R11491 Quant-iT™ RiboGreen® RNA Assay Kit *2,000 assays* 1 kit R11490 Quant-iT™ OliGreen™ ssDNA. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. 3 Q33120 39. Molecular Probes fluorescent and luminescent protein blot stains—Table 9. 2. Lambda DNA was used to generate the. 蛍光を用いたDNAの定量法の一つとしてInvitrogen™ Quant-iT™ PicoGreen™ dsDNA Assay Kit が利用されています。. Materials & Equipment Required But Not Provided • Pipettors - 5 L - 1200 L. 1. Further to the Quant-iT dsAssay kit (PicoGreen®), the Qubit dsDNA HS Assay kit offers an additional option for quantify-ing dsDNA samples of low concentration on the BioSpec-trometer fluorescence with high specificity and sensitivity. SpectraMax Plus Spectrophotometer (Molecular Devices) and Picogreen quantitation was performed using Quant-iT™ PicoGreen® dsDNA Assay Kit From Molecular Probes (Invitrogen). 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Quantitation using Quant-iT Assay Kits. Rapidly detect as little as 0. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. The HS (high sensitivity) assays quantitate the lowest levels of analyte, the BR (broad range) assays do so across a wider range, and the RNA XR. 5 µL of each extracted sample was used for Picogreen analysis. The PG reagent from the Quant-iT TM PicoGreen TM dsDNA Assay Kit (Invitrogen) was prepared immediately before use by making a 1:200 dilution of the PG in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7. 9, 10 The Pico-Green assay is sensitive to 25 pg dsDNA/mL, which is 400-fold more sensitive than Hoechst 33258 dye. Q33120) • Quant-iT dsDNA Assay Kit, broad range (Cat. lambda DNA standard 100ng/uL (in kit) PicoGreen reagent (in kit) 20x TE buffer (in kit) HPLC water; PicoGreen Prep. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The PicoGreen reagent was freshly prepared by diluting 15 μL reagent with 2 mL of TE buffer (~ 133X) and stored in the dark for no longer than 2 hours. DNA standards (λ DNA) and DNA samples were stained using a Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen, Eugene, OR, USA). Samples ranging in concentration from 0 ng/mL to 1,000 ng/mL were measured in quadruplicate, and the results are shown from the same data plotted in two different ways (Tables 2 and 3). After the reagent thawed, it is advisable to briefly vortex the tube toPlasma levels of cfDNA were measured using a fluorometric assay for double-stranded DNA Quant-iT PicoGreen dsDNA Assay kit (Invitrogen) following the manufacturer’s instructions. 20pg/µl for Quant-iT PicoGreen® (1µl of sample in a 200µl assay volume). Quant-iT dsDNA BR buffer (Component B) 250 ml c. Picogreen assays are compatible with most microplate readers and fluorometers. 3,201 3. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Bioz Stars score: 86/100, based on 1 PubMed citations. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Below are some easy steps to perform quantification in plates with Pico488 quantification kit by Lumiprobe (analog of Quant-iT® PicoGreen® dsDNA Assay Kit). The assay is linear over three orders of magnitude and has little sequence. Note that the assay volume is dependent on the instrument used to measure fl uorescence; with a microplate reader and a 96-well microplate, the assay volume is reduced to 200 μL and 2,000 assays are possible. ) Add PicoGreen to DNA samples Add 100 1x PicoGreen to each well of the DNA/standard plate without contaminating tips. You will need 100 ul diluted PicoGreen per well containing 100 ul sample. Highly selective for dsDNA over ssDNA or RNA. Reagents – The Quant-IT™ PicoGreen® kit was purchased from Thermo Fisher Scientific. Within two years of follow-up, the primary endpoint defined as nonfatal myocardial infarction, stroke or transient ischemic attack, cardiovascular death, and >80% target vessel restenosis occurred in 34 patients (43%). The following protocol is intended for the quantification of double-stranded DNA using Quant-iT™PicoGreen® dsDNA Assay Kit (ThermoFisher). dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. The kit contains. 0 fluorometer according to the manufacturer’s protocols; a. Qubit® dsDNA BR Assay Kit. Rapidly detect as little as 0. dsDNA in cytoplasmic fractions was quantified using the Quant-iT PicoGreen dsDNA. Thermo Fisher quant it picogreen dsdna quantitation assay kit. 2. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. Figure 2. 5mL of TE x 5uL PicoReagent. The user guide provides detailed information on the product description, component concentration, storage, assay protocol, and safety data sheets of the Quant-iTTM PicoGreenTM dsDNA Reagent and Kit. 5mL. 2023-11. Average 86 stars, based on 1. The high sensitivity kit is superior to the PicoGreen™ or Quant-iT™ dsDNA detection kits by having aPicogreen是一种极为灵敏的荧光核酸染料，其仅在与DNA双链结合后才发出荧光，且所产生的荧光与DNA浓度呈正比，因而可用于精确测量多个来源的dsDNA，包括基因组DNA、病毒DNA、PCR扩增产物、测序文库等。 本产品Picogreen dsDNA Quantitation Reagent定量具有以下优势：①灵敏度高：可检测低至200 pg的dsDNA，节省. Quantitation Range. Helixyte™ Green exhibits large fluorescence enhancement upon binding to dsDNA. • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. Sequencing libraries were built using Illumina's TruSeq Nano DNA LT Library Prep Kit, and the NovaSeq 6000 SP Reagent Kit (500 cycles) was used. The assay is highly selective for double-stranded DNA over RNA, and in the range of 4–1,000 ng, the fluorescence signal is linear with DNA (see “Assay selectivity” on page 1). Specifications Documents Scientific Resources Application Notes Quant-iT PicoGreen dsDNA AssayキットおよびdsDNA試薬を使用すると、幅広いダイナミックレンジでdsDNA濃度測定を正確かつ精密に行うことができます。. P11496). • Significantly more sensitive than UV absorbance readings, saving precious sample amounts. The assay is linear over three orders of magnitude and has little sequence dependence, allowing you to accurately measure DNA from many sources, including genomic DNA, viral DNA. 20–1,000 ng Qubit RNA BR Assay Kit 5–100 ng Qubit RNA HS Assay Kit Varioskan LUX Multimode Microplate Reader 2–1,000 ng Quant-iT dsDNA BR Assay Kit 0. クイックオーダー. Rapidly detect as little as 0. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. This kit contains. Price from $9. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. 7 Q33232 4 Green features Less waste and use of fewer resources With the Qubit and Quant-iT 1X dsDNA HS and 1X dsDNA BR Assay Kits, instead of supplying the assay reagent and dilution buffer in separate bottles as was done with the. The libraries were sequenced using the Illumina HiSeq 2500. Optimized for fluorescence microplate readers. Abstract. 0 fluorometer according to the manufacturer’s protocols; a sample volume of 1 μl was. Rapidly detect as little as 0. Figure 2. Total amount of 1X TE per assay will be 200 μl per well which includes the amount of TE used to dilute the PicoGreen reagent. Using 20x TE buffer make 1x solution. The DNA standard was Lambda DNA included in the Quant-iT PicoGreen dsDNA Assay Kit (ThermoFisher). 86100 stars. 005% Triton X-100, Tween-20, or Brij. 2023-11. • Suitable for use in 96- and 384-well plate formats. The standard PicoGreen assay protocol is also simpler than the Hoechst 33258 method, because a single concentration of the PicoGreen. 0 ml 200x in DMSO b. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free nucleotides, using. Samples should be free of RNA contamination and the use of the Qiagen Reaction Cleanup Kit (Qiagen) is recommended. The Quant-iT family of assay kits—and the related Invitrogen Qubit family of assay kits designed for use with the Invitrogen Qubit 2. The original sequencing data. 특징: Quanti-iT Picogreen을 이용하면 낮은 농도의 DNA 측정에 유리. Catalog Number. Rapidly detect as little as 0. UV흡광 결과보다 민감도 우수. Quant-iT PicoGreen dsDNA Assay Kit: Quantitation Range: 50 pg to 2 µg: Shipping Condition: Room Temperature: Detection Method: Fluorescence: Unit Size: 10 x 100 µL kit: Save to list Add to cart. 5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format. Quant It Picogreen Dsdna Staining Kit, supplied by Thermo Fisher, used in various techniques. DNA standard curve. 10pg/µl vs. Accuracy of the Qubit dsDNA HS assay compared to the Quant-iT PicoGreen dsDNA assay plotted on two different types of curves. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA, and free. ZERO BIAS - scores, article reviews, protocol conditions and moreAchieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The DNA sample used for aliquoting was extracted from soil using the DNeasy PowerSoil Kit (Qiagen). Working PicoGreen reagent was prepared by diluting the concentrated DMSO-PicoGreen stock solution, provided in the PicoGreen kit, 1:200 with TE according to the kit instructions. Qubit DNA and RNA assays are available with different quantification ranges. Picogreen Assay for DNA quantification: Which one is better to go for, a fresh. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. The Quant-iT™ PicoGreen® DNA assay is the oldest assay and the most general-purpose. Quantification of cfDNA was performed using a TECAN Safire plate reader (96-well format) in combination with the Quant-iT ™ PicoGreen ™ dsDNA Assay Kit (Molecular Probes, Invitrogen; cat #P11496, lot #1911829) according to the manufacturer’s instructions, with slight modifications. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. Catalog Number. Los ensayos Picogreen son compatibles con la mayoría de los lectores de microplacas y fluorómetros. 10 x 100 µL. 2–100 ng: 2-1,000 ng: 0. 作成者 LatB Staff 01. Bioz Stars score: 86/100, based on 1 PubMed citations. The fluorescence intensity of PicoGreen was measured to determine the. Quant-iT PicoGreen dsDNA Assay Kit. Quant-iT ™ 1X dsDNA Assay Kit, Broad-Range 4 ng−2 μg 1,000 reactions Q33267The supernatant containing the cytosolic extract was either immediately used or stored at − 80 °C until use. • Specific for dsDNA in the presence of RNA. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. Quantification and normalization of double stranded DNA with minimal set-up time. Simply dilute the reagent 1:200, load 200 µL into the wells of a microplate, add 1–20 µL sample, mix, then read the. Quant-iT PicoGreen dsDNA Assay Kit. Regulations & Guidance; Manuals 0 0. Picogreen assays are compatible with most microplate readers and fluorometers. After quantitation, the purified amplicons from each reaction mixture were mixed at equimolar ratios based on concentration and subjected to. Briefly, 2 µL of sample was diluted in 498 µL TE. Sample cDNA concentration was measured using the Quant-iT PicoGreen dsDNA assay kit and black Nunc F96 MicroWell polystyrene plates (ThermoFisher Scientific) according to the manufacturer’s. Determination of DNA Yield. The range of linearity for DNA concentration measurements. 25 pg/uL of dsDNA, even in the presence of ssDNA, RNA,. Dynamic range and sensitivity of the Quant-iTTM PicoGreen® dsDNA assay. • Easy to use—just add the dye working solution to the sample, incubate five minutes, and read. The QuantiFluor® dsDNA Dye offers greater sensitivity over absorbance at 260nm (NanoDrop®) low-concentration samples, and better or equal sensitivity performance when compared to PicoGreen®. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 µL assay volume) with a. Easy to use UV absorbance alternative assay that can be. 99 to $1999. Quant It Picogreen Dsdna Assay Kit Manual - 114,697 . Table 2 summarizes results of the. Replicates of four were added to a 96-well plate using 2 μL volumes for rows A through D (beige) and 10 μL volumes for rows E through H (blue). The reagent Quant-iT PicoGreen was added to all wells and the samples were incubated for 5 min at room. 5 ng/ml ~ 1,000 ng/ml 까지 정비례. PicoGreen assay. Quant-iT PicoGreen dsDNA Assay Kit a. Achieve precise and accurate dsDNA concentration measurements over a broad dynamic range with the Quant-iT Picogreen dsDNA Assay kits and dsDNA reagents. This assay is designed for use with fluorescence microplate readers. Preparing the Reagent On the day of the experiment, prepare an aqueous working solution of the Quant-iT™ The Quant-iT™ dsDNA High-Sensitivity Assay Kit makes DNA quantification easy and accurate. An aqueous working solution of PG reagent was freshly prepared as a 1:200 dilution of the concentrated DMSO solution to 1x TE buffer (10 mM Tris–HCl, 1 mM EDTA, pH 7. ITSkabir). It must be completely liquified before use by allowing it to come to room temperature. Technical Support Customer Service. The normalized DNA was aliquoted into multiple tubes at 50µL volume. Quant-iT PicoGreen dsDNA Assay Kit. • Suitable for use in 96- and 384-well plate formats. Skip to main list 美谷分子仪器（上海）有限公司. If your are using plastic cuvettes or assay plates, reduce this problem by including 0.